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Addgene

pMT1004
(Plasmid #8613)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 8613 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    partial pUC19
  • Backbone size w/o insert (bp) 1829
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    binase, barstar
  • Species
    Bacillus intermedius
  • Insert Size (bp)
    1857
  • Mutation
    as pMT1002
  • GenBank ID
    X53697

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer na
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

As pMT1002 with the structural gene for mature barnase replaced by that of binase, the barnase homolog from B. intermedius. Binase gene from B. intermedius strain 7P.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMT1004 was a gift from Robert Hartley (Addgene plasmid # 8613 ; http://n2t.net/addgene:8613 ; RRID:Addgene_8613)
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