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Addgene

GIV-FLAG L249P (LP)
(Plasmid #86097)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86097 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p3XFLAG-CMV-14
  • Backbone size w/o insert (bp) 6300
  • Total vector size (bp) 11910
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GIV/Girdin
  • Alt name
    GIV
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5610
  • Mutation
    L249P
  • GenBank ID
    BAE44387.1, AB201172.1
  • Entrez Gene
    CCDC88A (a.k.a. APE, GIRDIN, GIV, GRDN, HkRP1, KIAA1212, PEHO, PEHOL)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFlag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hGH-PA-R (5'-CCAGCTTGGTTCCCAATAGA-3')
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GIV-FLAG L249P (LP) was a gift from Pradipta Ghosh (Addgene plasmid # 86097 ; http://n2t.net/addgene:86097 ; RRID:Addgene_86097)

  • For your References section:

    AMP-activated protein kinase fortifies epithelial tight junctions during energetic stress via its effector GIV/Girdin. Aznar N, Patel A, Rohena CC, Dunkel Y, Joosen LP, Taupin V, Kufareva I, Farquhar MG, Ghosh P. Elife. 2016 Nov 4;5. pii: e20795. doi: 10.7554/eLife.20795. 10.7554/eLife.20795 PubMed 27813479
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