IL2Rα-EGFP
(Plasmid #86055)

• Purpose: A mammalian expression plasmid encoding IL2Rα (interleukin-2 receptor subunit alpha) with C-terminus EGFP.
• Depositing Lab: Lei Lu
• Publication: Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-3934. Epub 2016 Sep 15.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5500
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Interleukin-2 receptor subunit alpha
• Species: H. sapiens (human)
• Entrez Gene: IL2RA (a.k.a. CD25, IDDM10, IL2R, IMD41, TCGFR, p55)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry