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sFlt1-e15a V5
(Plasmid #86045)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86045 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    TOPO Directional
  • Backbone manufacturer
    thermofisher
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human soluble Flt1 isoform e15a
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2342
  • Mutation
    isoform e15a (terminates in exon 15a)
  • Entrez Gene
    FLT1 (a.k.a. FLT, FLT-1, VEGFR-1, VEGFR1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • 6xHis (C terminal on backbone)
    • V5 (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sFlt1-e15a V5 was a gift from Christie Thomas (Addgene plasmid # 86045 ; http://n2t.net/addgene:86045 ; RRID:Addgene_86045)

  • For your References section:

    A recently evolved novel trophoblast-enriched secreted form of fms-like tyrosine kinase-1 variant is up-regulated in hypoxia and preeclampsia. Thomas CP, Andrews JI, Raikwar NS, Kelley EA, Herse F, Dechend R, Golos TG, Liu KZ. J Clin Endocrinol Metab. 2009 Jul;94(7):2524-30. doi: 10.1210/jc.2009-0017. Epub 2009 Mar 31. 10.1210/jc.2009-0017 PubMed 19336504
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