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Addgene

OccludinMut_Flag
(Plasmid #86043)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86043 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFlag CMV-6C
  • Backbone manufacturer
    SigmaAldrich
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    OccludinMut
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1601
  • Mutation
    PPPY domain at AA9-12 to PAAY; I52T and Q365P
  • GenBank ID
    NM_002538.3
  • Entrez Gene
    OCLN (a.k.a. BLCPMG, PPP1R115, PTORCH1)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    OccludinMut_Flag was a gift from Christie Thomas (Addgene plasmid # 86043 ; http://n2t.net/addgene:86043 ; RRID:Addgene_86043)

  • For your References section:

    Nedd4-2 interacts with occludin to inhibit tight junction formation and enhance paracellular conductance in collecting duct epithelia. Raikwar NS, Vandewalle A, Thomas CP. Am J Physiol Renal Physiol. 2010 Aug;299(2):F436-44. doi: 10.1152/ajprenal.00674.2009. Epub 2010 May 26. 10.1152/ajprenal.00674.2009 PubMed 20504882
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