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Addgene

hNedd4-2 CS
(Plasmid #86041)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86041 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4 HisMax
  • Backbone manufacturer
    thermofisher
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hNeddL3 with C to S mutation in HECT domain
  • Alt name
    NM_001144967.2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3035
  • Mutation
    C942S mutation in HECT domain
  • Entrez Gene
    NEDD4 (a.k.a. NEDD4-1, RPF1)
  • Promoter CMV
  • Tag / Fusion Protein
    • His and Xpress (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hNedd4-2 CS was a gift from Christie Thomas (Addgene plasmid # 86041 ; http://n2t.net/addgene:86041 ; RRID:Addgene_86041)

  • For your References section:

    Nedd4-2 interacts with occludin to inhibit tight junction formation and enhance paracellular conductance in collecting duct epithelia. Raikwar NS, Vandewalle A, Thomas CP. Am J Physiol Renal Physiol. 2010 Aug;299(2):F436-44. doi: 10.1152/ajprenal.00674.2009. Epub 2010 May 26. 10.1152/ajprenal.00674.2009 PubMed 20504882
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