hSgk1_v2 Flag
(Plasmid
#86040)
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Purposefull length human Sgk1_i2 with Flag tag
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 86040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.1
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Backbone manufacturerthermofisher
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAlternate transcript v2 of human Sgk1
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SpeciesH. sapiens (human)
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Insert Size (bp)1637
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Mutationv2
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Entrez GeneSGK1 (a.k.a. SGK)
- Promoter CMV
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Tag
/ Fusion Protein
- FLAG (C terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-Rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hSgk1_v2 Flag was a gift from Christie Thomas (Addgene plasmid # 86040 ; http://n2t.net/addgene:86040 ; RRID:Addgene_86040) -
For your References section:
An evolutionarily conserved N-terminal Sgk1 variant with enhanced stability and improved function. Raikwar NS, Snyder PM, Thomas CP. Am J Physiol Renal Physiol. 2008 Nov;295(5):F1440-8. doi: 10.1152/ajprenal.90239.2008. Epub 2008 Aug 27. 10.1152/ajprenal.90239.2008 PubMed 18753299