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Addgene

hSgk1_v3 Flag
(Plasmid #86039)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86039 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    thermofisher
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Alternate transcript v3 of human Sgk1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1394
  • Mutation
    v3; S313G
  • Entrez Gene
    SGK1 (a.k.a. SGK)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hSgk1_v3 Flag was a gift from Christie Thomas (Addgene plasmid # 86039 ; http://n2t.net/addgene:86039 ; RRID:Addgene_86039)

  • For your References section:

    A regulated NH2-terminal Sgk1 variant with enhanced function is expressed in the collecting duct. Raikwar NS, Liu KZ, Thomas CP. Am J Physiol Renal Physiol. 2012 Dec 1;303(11):F1527-33. doi: 10.1152/ajprenal.00191.2012. Epub 2012 Oct 3. 10.1152/ajprenal.00191.2012 PubMed 23034940
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