hFlt1 V767A
(Plasmid #86038)

• Purpose: human VEGFR1 with V to A mutation at position 767
• Depositing Lab: Christie Thomas
• Publication: Raikwar et al Exp Cell Res. 2016 May 15;344(1):103-11. doi: 10.1016/j.yexcr.2016.03.020. Epub 2016 Mar 23.

Backbone

• Vector backbone: pCMV6 Entry
• Backbone manufacturer: Origene
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human Flt1 V767A
• Species: H. sapiens (human)
• Insert Size (bp): 4286
• Mutation: V767A
• Entrez Gene: FLT1 (a.k.a. FLT, FLT-1, VEGFR-1, VEGFR1)
• Promoter: CMV
• Tags / Fusion Proteins:
  • FLAG (C terminal on backbone)
  • HA (please see depositor comments below) (N terminal on insert)
  • Myc (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: hGH-PA-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Since the signal sequence (the first few AA) for Flt1 is cleaved off , the HA tag was placed between AA30-31. The final mature protein has the HA tag N-terminal to the protein.