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Addgene

hFLT1 delta CTD
(Plasmid #86036)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86036 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV6 Entry
  • Backbone manufacturer
    Origene
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Flt1 delta CTD
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2567
  • Mutation
    deta CTD
  • Entrez Gene
    FLT1 (a.k.a. FLT, FLT-1, VEGFR-1, VEGFR1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • FLAG (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hFLT1 delta CTD was a gift from Christie Thomas (Addgene plasmid # 86036 ; http://n2t.net/addgene:86036 ; RRID:Addgene_86036)

  • For your References section:

    N-terminal cleavage and release of the ectodomain of Flt1 is mediated via ADAM10 and ADAM 17 and regulated by VEGFR2 and the Flt1 intracellular domain. Raikwar NS, Liu KZ, Thomas CP. PLoS One. 2014 Nov 11;9(11):e112794. doi: 10.1371/journal.pone.0112794. eCollection 2014. PONE-D-14-21798 [pii] PubMed 25387128
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