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Addgene

hFLT1 delta 754-763
(Plasmid #86035)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86035 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV6 Entry
  • Backbone manufacturer
    Origene
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human Flt1 delta754-763 aa
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4253
  • Mutation
    delta 754-763
  • Entrez Gene
    FLT1 (a.k.a. FLT, FLT-1, VEGFR-1, VEGFR1)
  • Promoter CMV
  • Tags / Fusion Proteins
    • FLAG (C terminal on backbone)
    • HA (N terminal on backbone)
    • Myc (C terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hFLT1 delta 754-763 was a gift from Christie Thomas (Addgene plasmid # 86035 ; http://n2t.net/addgene:86035 ; RRID:Addgene_86035)

  • For your References section:

    Ectodomain cleavage of FLT1 regulates receptor activation and function and is not required for its downstream intracellular cleavage. Raikwar NS, Liu KZ, Thomas CP. Exp Cell Res. 2016 May 15;344(1):103-11. doi: 10.1016/j.yexcr.2016.03.020. Epub 2016 Mar 23. 10.1016/j.yexcr.2016.03.020 PubMed 27017929
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