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Addgene

FLT1-KDR
(Plasmid #86034)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86034 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV6 Entry
  • Backbone manufacturer
    Origene
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human VEGFR1-VEGFR2 hybrid
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4940
  • Entrez Gene
    FLT1 (a.k.a. FLT, FLT-1, VEGFR-1, VEGFR1)
  • Entrez Gene
    KDR (a.k.a. CD309, FLK1, VEGFR, VEGFR2)
  • Tags / Fusion Proteins
    • HA (N terminal on backbone)
    • t-GFP (C terminal on backbone)

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLT1-KDR was a gift from Christie Thomas (Addgene plasmid # 86034 ; http://n2t.net/addgene:86034 ; RRID:Addgene_86034)

  • For your References section:

    Ectodomain cleavage of FLT1 regulates receptor activation and function and is not required for its downstream intracellular cleavage. Raikwar NS, Liu KZ, Thomas CP. Exp Cell Res. 2016 May 15;344(1):103-11. doi: 10.1016/j.yexcr.2016.03.020. Epub 2016 Mar 23. 10.1016/j.yexcr.2016.03.020 PubMed 27017929
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