Skip to main content
Addgene

pCDNAZeo(-)HSC73AS
(Plasmid #86031)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86031 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    PCDNA Zeo(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6145
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    heat shock cognate protein 70 (hsc70)
  • Alt name
    HSC 70 , HSC 73, HSPA8
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1145
  • GenBank ID
    NM_006597.5
  • Entrez Gene
    HSPA8 (a.k.a. HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Pst1 (unknown if destroyed)
  • 3′ cloning site Hind III (unknown if destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. A.M. Cuervo, Tufts University

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

HSC73 sequence is inserted in an inverted orientation (Antisense construct)

Addgene NGS results found L394S within the HSC73 translation compared to NM_006597.5; however plasmid does encode mRNA which is functional.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNAZeo(-)HSC73AS was a gift from Janice Blum (Addgene plasmid # 86031 ; http://n2t.net/addgene:86031 ; RRID:Addgene_86031)

  • For your References section:

    Lamp-2a facilitates MHC class II presentation of cytoplasmic antigens. Zhou D, Li P, Lin Y, Lott JM, Hislop AD, Canaday DH, Brutkiewicz RR, Blum JS. Immunity. 2005 May;22(5):571-81. 10.1016/j.immuni.2005.03.009 PubMed 15894275
Related items:
Commonly requested with: