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Addgene

pCDNAZeo(-)HSC73
(Plasmid #86030)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86030 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDNAZeo(-)
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5000
  • Total vector size (bp) 6145
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Heat-shock cognate protein 73
  • Alt name
    HSC73, HSC70
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1145
  • Entrez Gene
    HSPA8 (a.k.a. HEL-33, HEL-S-72p, HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, LAP-1, LAP1, NIP71)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer m13 reverse
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. A.M. Cuervo, Tufts University

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results found L394S within the HSC73 translation compared to NM_006597.5; however plasmid encodes mRNA which is functional.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNAZeo(-)HSC73 was a gift from Janice Blum (Addgene plasmid # 86030 ; http://n2t.net/addgene:86030 ; RRID:Addgene_86030)

  • For your References section:

    Lamp-2a facilitates MHC class II presentation of cytoplasmic antigens. Zhou D, Li P, Lin Y, Lott JM, Hislop AD, Canaday DH, Brutkiewicz RR, Blum JS. Immunity. 2005 May;22(5):571-81. 10.1016/j.immuni.2005.03.009 PubMed 15894275
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