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pGL4.23 A82V/T230A/D637G 2014 EBOV + Mucin-Like Domain
(Plasmid #86020)

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Item Catalog # Description Quantity Price (USD)
Plasmid 86020 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGL4.23
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2031
  • Total vector size (bp) 5198
  • Modifications to backbone
    CMV IE promoter/enhancer inserted upstream of minimal promoter
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Makona Ebola Virus Glycoprotein
  • Alt name
    EBOV GP
  • Alt name
    EBOV Makona GP
  • Species
    Zaire ebolavirus - Makona
  • Insert Size (bp)
    2031
  • Mutation
    Changed alanine 82 to valine, threonine 230 to alanine, and aspartic acid 637 to glycine
  • GenBank ID
    KJ660346.2 AHX24649.2
  • Promoter CMV IE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV F
  • 3′ sequencing primer SV40 pA R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.23 A82V/T230A/D637G 2014 EBOV + Mucin-Like Domain was a gift from Jeremy Luban & Pardis Sabeti (Addgene plasmid # 86020 ; http://n2t.net/addgene:86020 ; RRID:Addgene_86020)

  • For your References section:

    Ebola Virus Glycoprotein with Increased Infectivity Dominated the 2013-2016 Epidemic. Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber M, Rambaut A, Andersen KG, Sabeti PC, Luban J. Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 10.1016/j.cell.2016.10.014. 10.1016/j.cell.2016.10.014 PubMed 27814506
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