pJ3H PTP-1B
(Plasmid #8601)

• Depositing Lab: Ben Neel
• Publication: Venable et al J Biol Chem. 2000 Jun 16. 275(24):18318-26.

Backbone

• Vector backbone: pJ3H
• Backbone size w/o insert (bp): 3500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PTP-1B
• Alt name: PTPN1
• Species: H. sapiens (human)
• Insert Size (bp): 1400
• Mutation: K103R
• Entrez Gene: PTPN1 (a.k.a. PTP1B)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: SV40pro-F

Resource Information

• A portion of this plasmid was derived from a plasmid made by: J. Chernoff, Fox Chase Cancer Center
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry