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Addgene

pET28a SUMO Nup53xl 162-267
(Plasmid #86002)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86002 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28a
  • Backbone manufacturer
    Novagene
  • Backbone size (bp) 5300
  • Modifications to backbone
    removal of the N-Terminal HIS6 tag, addition of teh MISTIC sequence upstream of the MCS
  • Vector type
    Bacterial Expression
  • Tag / Fusion Protein
    • SUMO (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    we received the original MISTIC seuqnce from Senyon Choe, Salk Institute

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a SUMO Nup53xl 162-267 was a gift from Wolfram Antonin (Addgene plasmid # 86002 ; http://n2t.net/addgene:86002 ; RRID:Addgene_86002)
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