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Addgene

pSI HA Nup53xl
(Plasmid #86000)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86000 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSI
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Xenopus laevis Nup53
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    960
  • Mutation
    codon optimzed for bacterial expression
  • Entrez Gene
    nup35.L (a.k.a. XELAEV_18044791mg, nup35, nup53)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSI HA Nup53xl was a gift from Wolfram Antonin (Addgene plasmid # 86000 ; http://n2t.net/addgene:86000 ; RRID:Addgene_86000)

  • For your References section:

    Dimerization and direct membrane interaction of Nup53 contribute to nuclear pore complex assembly. Vollmer B, Schooley A, Sachdev R, Eisenhardt N, Schneider AM, Sieverding C, Madlung J, Gerken U, Macek B, Antonin W. EMBO J. 2012 Oct 17;31(20):4072-84. doi: 10.1038/emboj.2012.256. Epub 2012 Sep 7. 10.1038/emboj.2012.256 PubMed 22960634
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