pET28a MISTIC
(Plasmid #85999)

• Purpose: bacterial expression of transmembrane proteins using N-terminal MISTIC and C-terminal HIS6 tag
• Depositing Lab: Wolfram Antonin
• Publication: Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi: 10.1038/emboj.2012.256. Epub 2012 Sep 7.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagene
• Backbone size w/o insert (bp): 5300
• Modifications to backbone: removal of the N-Terminal HIS6 tag, addition of the MISTIC sequence upstream of the MCS
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: N/A
• Insert Size (bp): 960
• Mutation: codon optimzed for bacterial expression
• Promoter: T7
• Tags / Fusion Proteins:
  • MISTIC (N terminal on insert)
  • HIS6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)
• 5′ sequencing primer: lac
• 3′ sequencing primer: pETrv

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry