pET28a SUMO Nup53xl 1-320
(Plasmid #85998)

• Purpose: bacterial expression of his6 and SUMO tagged Xenopus laevis Nup53
• Depositing Lab: Wolfram Antonin
• Publication: Vollmer et al EMBO J. 2012 Oct 17;31(20):4072-84. doi: 10.1038/emboj.2012.256. Epub 2012 Sep 7.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagene
• Backbone size w/o insert (bp): 5300
• Modifications to backbone: removal of the N-Terminal HIS6 tag, addition of teh MISTIC sequence upstream of the MCS
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Xenopus laevis Nup53
• Species: X. laevis (frog)
• Insert Size (bp): 960
• Mutation: codon optimzed for bacterial expression
• Entrez Gene: nup35.L (a.k.a. XELAEV_18044791mg, nup35, nup53)
• Tag / Fusion Protein:
  • SUMO (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Nde (not destroyed)
• 3′ cloning site: Xho (not destroyed)
• 5′ sequencing primer: pETfw
• 3′ sequencing primer: pEtrv

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry