p112A1NE
(Plasmid
#85983)
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Purpose(Empty Backbone) For construction of cDNA expression library.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85983 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep112A1NE
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Vector typeYeast Expression
- Promoter Adh1
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p112A1NE was a gift from Wolf Frommer (Addgene plasmid # 85983 ; http://n2t.net/addgene:85983 ; RRID:Addgene_85983) -
For your References section:
Isolation and characterization of a sucrose carrier cDNA from spinach by functional expression in yeast. Riesmeier JW, Willmitzer L, Frommer WB. EMBO J. 1992 Dec;11(13):4705-13. PubMed 1464305
