pCR8/GW:U6
(Plasmid
#85981)
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PurposeGolden Gate Level 0 cassette encoding the U6 promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85981 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCR8/GW/TOPO
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Backbone manufacturerThermo Fisher
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Vector typeCRISPR ; Diatom (T. pseudonana)
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameT. pseudonana U6 promoter
- Promoter none
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13-Rev (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR8/GW:U6 was a gift from Thomas Mock (Addgene plasmid # 85981 ; http://n2t.net/addgene:85981 ; RRID:Addgene_85981) -
For your References section:
Editing of the urease gene by CRISPR-Cas in the diatom Thalassiosira pseudonana. Hopes A, Nekrasov V, Kamoun S, Mock T. Plant Methods. 2016 Nov 24;12:49. doi: 10.1186/s13007-016-0148-0. eCollection 2016. 10.1186/s13007-016-0148-0 PubMed 27904648
