pGEX-2T SHP1 WT
(Plasmid #8594)

• Depositing Lab: Ben Neel
• Publication: Plutzky et al Proc Natl Acad Sci U S A 1992 Feb 1;89(3):1123-7.

Backbone

• Vector backbone: pGEX-2T
• Backbone size w/o insert (bp): 4948
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SHP1
• Alt name: SH-PTP1
• Alt name: PTPN6
• Species: H. sapiens (human)
• Insert Size (bp): 2100
• Entrez Gene: PTPN6 (a.k.a. HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, SHP1)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: pGEX 5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Linker between GST and SHP1 13aa of 5' UTR was used. (leu-his-ile-phe-ser-glu-asn-pro-arg-met of SHP1)