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PurposeExpressed N-term tagged APEX2 on HMOX1 in mammalian cell
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85824 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
- Backbone size w/o insert (bp) 5382
- Total vector size (bp) 7053
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameV5-APEX2-HMOX1
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Alt nameHeme oxygenase 1
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)1671
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GenBank IDNM_002133.2.
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Entrez GeneHMOX1 (a.k.a. HMOX1D, HO-1, HSP32, bK286B10)
- Promoter CMV-F
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Tags
/ Fusion Proteins
- V5 (N terminal on insert)
- APEX2 (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCDNA3_V5-APEX2-HMOX1 was a gift from Hyun-Woo Rhee (Addgene plasmid # 85824 ; http://n2t.net/addgene:85824 ; RRID:Addgene_85824) -
For your References section:
APEX Fingerprinting Reveals the Subcellular Localization of Proteins of Interest. Lee SY, Kang MG, Park JS, Lee G, Ting AY, Rhee HW. Cell Rep. 2016 May 11. pii: S2211-1247(16)30508-3. doi: 10.1016/j.celrep.2016.04.064. 10.1016/j.celrep.2016.04.064 PubMed 27184847
