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Addgene

pCMV5-hPPT1
(Plasmid #85815)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85815 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CMV
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    human PPT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    980
  • GenBank ID
    NM_000310
  • Entrez Gene
    PPT1 (a.k.a. CLN1, INCL, PPT)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV5-hPPT1 was a gift from Sandra Hofmann (Addgene plasmid # 85815 ; http://n2t.net/addgene:85815 ; RRID:Addgene_85815)

  • For your References section:

    Human recombinant palmitoyl-protein thioesterase-1 (PPT1) for preclinical evaluation of enzyme replacement therapy for infantile neuronal ceroid lipofuscinosis. Lu JY, Hu J, Hofmann SL. Mol Genet Metab. 2010 Apr;99(4):374-8. doi: 10.1016/j.ymgme.2009.12.002. Epub 2009 Dec 5. 10.1016/j.ymgme.2009.12.002 PubMed 20036592
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