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Addgene

pCI-neo-Flag-DHHS
(Plasmid #85813)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85813 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone size w/o insert (bp) 5500
  • Total vector size (bp) 7800
  • Modifications to backbone
    inserted 3x Flag tag
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZDHHC5 C134S mutant
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2200
  • GenBank ID
    NM_144887
  • Entrez Gene
    Zdhhc5 (a.k.a. 1110032A17Rik, AI451382, Zisp)
  • Tag / Fusion Protein
    • Flag (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-neo-Flag-DHHS was a gift from Sandra Hofmann (Addgene plasmid # 85813 ; http://n2t.net/addgene:85813 ; RRID:Addgene_85813)

  • For your References section:

    Systematic siRNA Screen Unmasks NSCLC Growth Dependence by Palmitoyltransferase DHHC5. Tian H, Lu JY, Shao C, Huffman KE, Carstens RM, Larsen JE, Girard L, Liu H, Rodriguez-Canales J, Frenkel EP, Wistuba II, Minna JD, Hofmann SL. Mol Cancer Res. 2015 Apr;13(4):784-94. doi: 10.1158/1541-7786.MCR-14-0608. Epub 2015 Jan 8. 10.1158/1541-7786.MCR-14-0608 PubMed 25573953
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