pAAVS1-Neo-CAG-M2rtTA-H2BmNeon (donorAB-mNeon)
(Plasmid #85799)

• Purpose: donorAB-Neon targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP
• Depositing Lab: Roland Friedel
• Publication: Tejero et al EBioMedicine. 2019 Apr 2. pii: S2352-3964(19)30209-9. doi: 10.1016/j.ebiom.2019.03.064.

Backbone

• Vector backbone: N/A
• Backbone size w/o insert (bp): 3961
• Total vector size (bp): 11844
• Vector type: Human Targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PPP1R12C
• Alt name: AAVS1-homol arms T2A-Neo CAG-rtTA-M2 tetO-H2B-mNeon
• Alt name: donor AB-mNeon
• Species: H. sapiens (human)
• Insert Size (bp): 7883
• Mutation: homology arms for knock-in into first intron
• Entrez Gene: PPP1R12C (a.k.a. AAVS1, LENG3, MBS85, p84, p85)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: ClaI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: T3

Resource Information

• Supplemental Documents:
  • donor AB vector series and targeting strategy

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid cloned by combining vectors obtained from Hockemeyer and Jaenisch (Addgene #60843) laboratories. Please note that some discrepancies were found between Addgene's quality control result and the depositor's full sequence. The depositor noted that these discrepancies do NOT affect plasmid function.