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PurposedonorAB targeting vector for human AAVS1 locus; knock-in of Dox controlled H2BGFP expression system
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 85798 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneN/A
- Backbone size w/o insert (bp) 3955
- Total vector size (bp) 11846
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Vector typeHuman Targeting
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePPP1R12C
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Alt nameAAVS1-homol arms T2A-Neo CAG-rtTA-M2 tetO-H2B-GFP
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Alt namedonor AB
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SpeciesH. sapiens (human)
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Insert Size (bp)7891
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Mutationhomology arms for knock-in into first intron
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Entrez GenePPP1R12C (a.k.a. AAVS1, LENG3, MBS85, p84, p85)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site HindIII (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T3 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Addgene Notes
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid cloned by combining vectors obtained from Hockemeyer and Jaenisch (Addgene #60843) laboratories
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAVS1-Neo-CAG-M2rtTA-H2BGFP (donor AB) was a gift from Roland Friedel (Addgene plasmid # 85798 ; http://n2t.net/addgene:85798 ; RRID:Addgene_85798) -
For your References section:
Gene signatures of quiescent glioblastoma cells reveal mesenchymal shift and interactions with niche microenvironment. Tejero R, Huang Y, Katsyv I, Kluge M, Lin JY, Tome-Garcia J, Daviaud N, Wang Y, Zhang B, Tsankova NM, Friedel CC, Zou H, Friedel RH. EBioMedicine. 2019 Apr 2. pii: S2352-3964(19)30209-9. doi: 10.1016/j.ebiom.2019.03.064. 10.1016/j.ebiom.2019.03.064 PubMed 30952620