pSkipFlox
(Plasmid #85797)

• Purpose: P. falciparum plasmid for expression of both diCre fragments from single casette using skip peptide
• Depositing Lab: Tobias Spielmann
• Publication: Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223.

Backbone

• Vector backbone: pGEM
• Vector type: Cre/Lox ; P. falciparum Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: split Cre recombinase
• Species: Synthetic
• Promoter: Pfcrt
• Tag / Fusion Protein:
  • FKBP, FRB*

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: crtfw
• 3′ sequencing primer: pARLrv

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry