pLyn-FRB-mCherry-nmd3-BSD
(Plasmid #85796)

• Purpose: Expression of PPM mislocaliser in P. falciparum
• Depositing Lab: Tobias Spielmann
• Publication: Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223.

Backbone

• Vector backbone: pGEM
• Vector type: P. falciparum Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PPM Mislocaliser
• Species: Synthetic
• Promoter: Pfnmd3
• Tag / Fusion Protein:
  • FRB*, mCherry

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: XmaI (not destroyed)
• 5′ sequencing primer: nmd3fw
• 3′ sequencing primer: pARLrv

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry