Skip to main content
Addgene

p1xNLS-FRB-mCherry-hsp86-BSD
(Plasmid #85795)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85795 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pGEM
  • Vector type
    P. falciparum Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    1xNLS Mislocaliser
  • Species
    Synthetic
  • Promoter Pfhsp86
  • Tag / Fusion Protein
    • FRB*, mCherry

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XmaI (not destroyed)
  • 5′ sequencing primer hsp86fw
  • 3′ sequencing primer pARLrv
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p1xNLS-FRB-mCherry-hsp86-BSD was a gift from Tobias Spielmann (Addgene plasmid # 85795 ; http://n2t.net/addgene:85795 ; RRID:Addgene_85795)

  • For your References section:

    A genetic system to study Plasmodium falciparum protein function. Birnbaum J, Flemming S, Reichard N, Soares AB, Mesen-Ramirez P, Jonscher E, Bergmann B, Spielmann T. Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223. 10.1038/nmeth.4223 PubMed 28288121
Related items:
Commonly requested with: