pSLI-N-sandwich-loxP(K13)
(Plasmid
#85793)
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PurposeP. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLI
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85793 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM
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Vector typeCre/Lox ; P. falciparum genomic integration
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Selectable markershuman DHFR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameKelch13
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SpeciesP. falciparum
- Promoter none
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Tag
/ Fusion Protein
- double 2xFKBP, GFP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site PmeI (not destroyed)
- 5′ sequencing primer pARLfw
- 3′ sequencing primer DHODHrv (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSLI-N-sandwich-loxP(K13) was a gift from Tobias Spielmann (Addgene plasmid # 85793 ; http://n2t.net/addgene:85793 ; RRID:Addgene_85793) -
For your References section:
A genetic system to study Plasmodium falciparum protein function. Birnbaum J, Flemming S, Reichard N, Soares AB, Mesen-Ramirez P, Jonscher E, Bergmann B, Spielmann T. Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223. 10.1038/nmeth.4223 PubMed 28288121
