pSLI-sandwich(0807600)
(Plasmid #85790)

• Purpose: P. falciparum integration plasmid to fuse endogenous genes with tags (double 2xFKBP tag) using SLI
• Depositing Lab: Tobias Spielmann
• Publication: Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223.

Backbone

• Vector backbone: pGEM
• Vector type: P. falciparum genomic integration
• Selectable markers: human DHFR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PF3D0807600 targeting region, 2xFKBP-GFP-2xFKBP-2TA-Neo-R
• Species: P. falciparum
• Promoter: none
• Tag / Fusion Protein:
  • 2xFKBP, GFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (unknown if destroyed)
• 3′ cloning site: AvrII (unknown if destroyed)
• 5′ sequencing primer: pARLfw
• 3′ sequencing primer: GFP-85rv

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry