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pSLI-2xFKBP-GFP(1463000)
(Plasmid #85789)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85789 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM
  • Vector type
    P. falciparum integration
  • Selectable markers
    human DHFR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PF3D7146300 targeting region, 2xFKBP-GFP-2TA-Neo-R
  • Species
    P. falciparum
  • Promoter none
  • Tag / Fusion Protein
    • 2xFKBP, GFP

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site AvrII (unknown if destroyed)
  • 5′ sequencing primer pARLfw
  • 3′ sequencing primer GFP-85rv
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSLI-2xFKBP-GFP(1463000) was a gift from Tobias Spielmann (Addgene plasmid # 85789 ; http://n2t.net/addgene:85789 ; RRID:Addgene_85789)

  • For your References section:

    A genetic system to study Plasmodium falciparum protein function. Birnbaum J, Flemming S, Reichard N, Soares AB, Mesen-Ramirez P, Jonscher E, Bergmann B, Spielmann T. Nat Methods. 2017 Mar 13. doi: 10.1038/nmeth.4223. 10.1038/nmeth.4223 PubMed 28288121
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