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Addgene

pN3FS-CYC1
(Plasmid #85783)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85783 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUG6
  • Backbone manufacturer
    Euroscarf
  • Vector type
    Yeast Expression, Cre/Lox
  • Selectable markers
    Nourseothricin Sulfate

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    loxP-NatMX-loxP-CYC1p-3FLAG
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Mutation
    *(see below)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer CGCGTCAGCGGGTGTTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Note: Addgene's quality control sequencing finds a P77L amino acid residue substitution in the Nourseothricin resistance gene, but this residue change is not thought to affect antibiotic selection.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pN3FS-CYC1 was a gift from Scott Briggs (Addgene plasmid # 85783 ; http://n2t.net/addgene:85783 ; RRID:Addgene_85783)

  • For your References section:

    N-ICE plasmids for generating N-terminal 3 x FLAG tagged genes that allow Inducible, Constitutive, or Endogenous expression in Saccharomyces cerevisiae. Zhang Y, Serratore ND, Briggs SD. Yeast. 2016 Dec 12. doi: 10.1002/yea.3226. 10.1002/yea.3226 PubMed 27943405
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