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Addgene

pK3F-PYK1
(Plasmid #85771)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85771 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUG6
  • Backbone manufacturer
    Euroscarf
  • Vector type
    Yeast Expression, Cre/Lox
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    loxP-KanMX-loxP-PYK1p-3FLAG
  • Species
    S. cerevisiae (budding yeast), Synthetic
  • Insert Size (bp)
    2428

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer Spt6 promoter
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pK3F-PYK1 was a gift from Scott Briggs (Addgene plasmid # 85771 ; http://n2t.net/addgene:85771 ; RRID:Addgene_85771)

  • For your References section:

    N-ICE plasmids for generating N-terminal 3 x FLAG tagged genes that allow Inducible, Constitutive, or Endogenous expression in Saccharomyces cerevisiae. Zhang Y, Serratore ND, Briggs SD. Yeast. 2016 Dec 12. doi: 10.1002/yea.3226. 10.1002/yea.3226 PubMed 27943405
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