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USPL1cat-pETM30
(Plasmid #85768)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85768 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETM-30
  • Backbone size w/o insert (bp) 5992
  • Total vector size (bp) 6904
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    USPL1, catalytic domain (aa 212-514)
  • Alt name
    C13orf22
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    912
  • Mutation
    catalytic domain (aa 212-514)
  • Entrez Gene
    USPL1 (a.k.a. C13orf22, D13S106E, bA121O19.1)
  • Promoter T7-lac
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • GST (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer pET-RP
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    USPL1cat-pETM30 was a gift from Frauke Melchior (Addgene plasmid # 85768 ; http://n2t.net/addgene:85768 ; RRID:Addgene_85768)

  • For your References section:

    Ubiquitin-specific protease-like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions. Schulz S, Chachami G, Kozaczkiewicz L, Winter U, Stankovic-Valentin N, Haas P, Hofmann K, Urlaub H, Ovaa H, Wittbrodt J, Meulmeester E, Melchior F. EMBO Rep. 2012 Oct;13(10):930-8. doi: 10.1038/embor.2012.125. Epub 2012 Aug 10. 10.1038/embor.2012.125 PubMed 22878415
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