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USPL1cat-C236S-pEYFP_C1
(Plasmid #85767)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85767 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEYFP_C1
  • Backbone size w/o insert (bp) 4692
  • Total vector size (bp) 5604
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    USPL1, catalytic domain (aa 212-514), catalytic inactive variant C236S
  • Alt name
    C13orf22
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    912
  • Mutation
    catalytic domain (aa 212-514), catalytic inactive variant C236S
  • Entrez Gene
    USPL1 (a.k.a. C13orf22, D13S106E, bA121O19.1)
  • Promoter CMV
  • Tag / Fusion Protein
    • EYFP (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pEGFP_FP
  • 3′ sequencing primer pEGFP_C2_RP
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    USPL1cat-C236S-pEYFP_C1 was a gift from Frauke Melchior (Addgene plasmid # 85767 ; http://n2t.net/addgene:85767 ; RRID:Addgene_85767)

  • For your References section:

    Ubiquitin-specific protease-like 1 (USPL1) is a SUMO isopeptidase with essential, non-catalytic functions. Schulz S, Chachami G, Kozaczkiewicz L, Winter U, Stankovic-Valentin N, Haas P, Hofmann K, Urlaub H, Ovaa H, Wittbrodt J, Meulmeester E, Melchior F. EMBO Rep. 2012 Oct;13(10):930-8. doi: 10.1038/embor.2012.125. Epub 2012 Aug 10. 10.1038/embor.2012.125 PubMed 22878415
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