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Addgene

pSM1
(Plasmid #85752)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85752 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDONR 221
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 4762
  • Vector type
    Bacterial Expression
  • Promoter N/A

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin, 25 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Dr Richard Johnson, AgResearch New Zealand

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there are some seqeunce discrepancies between Addgene's quality control sequences and the depositor's genbank file. The depositor noted that these discrepancies do NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSM1 was a gift from Damien Fleetwood (Addgene plasmid # 85752 ; http://n2t.net/addgene:85752 ; RRID:Addgene_85752)

  • For your References section:

    Efficient targeted mutagenesis in Epichloe festucae using a split marker system. Rahnama M, Forester N, Ariyawansa KG, Voisey CR, Johnson LJ, Johnson RD, Fleetwood DJ. J Microbiol Methods. 2016 Dec 22. pii: S0167-7012(16)30368-2. doi: 10.1016/j.mimet.2016.12.017. 10.1016/j.mimet.2016.12.017 PubMed 28017692
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