pEJS481-pCDest2-AcrE2-mTagBFP2-IRES
(Plasmid #85749)

• Purpose: Expresses anti-CRISPR protein from Type I-E and mTagBFP2
• Depositing Lab: Erik Sontheimer
• Publication: Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. doi: 10.1016/j.cell.2016.11.017. Epub 2016 Dec 8.

Backbone

• Vector backbone: pCSDest2
• Total vector size (bp): 7119
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Type I-E anti-CRISPR
• Species: Pseudomonas aeruginosa
• Insert Size (bp): 252
• Promoter: CMV

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATTTAGGTGACACTATAGA

Gene/Insert 2

• Gene/Insert name: mTagBFP2
• Species: Synthetic
• Insert Size (bp): 711
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGC AAA TGG GCG GTA GGC GTG
• 3′ sequencing primer: CAG GAA ACA GCT ATG AC

Resource Information

• Supplemental Documents:
  • p481-pCDest-AcrE2 BFP2 IRES.gb
• A portion of this plasmid was derived from a plasmid made by: pIRES-mTagBFP2 is a gift from David Grünwald. Type I-E anti-CRISPR protein (AcrE2) is described by Pawluk et al. (2014).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Pawluk, A., Bondy-Denomy, J., Cheung, V.H., Maxwell, K.L., and Davidson, A.R. (2014). A new group of phage anti-CRISPR genes inhibits the type I-E CRISPR-Cas system of Pseudomonas aeruginosa. MBio 5, e00896.