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Purpose(Empty Backbone) tetracyline-inducible lentiviral vector with no insert for use in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85747 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneFUW
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Backbone manufacturerRudolf Jaenisch
- Backbone size (bp) 9537
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Modifications to backbonepuro resistance added
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Vector typeLentiviral
- Promoter CMV
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Selectable markersPuromycin
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Tag
/ Fusion Protein
- none
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Growth instructionsprone to rearrangement
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Copy numberUnknown
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer fuwF (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
EcoRI
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TetO-FUW-pgk-puro was a gift from Emily Dykhuizen (Addgene plasmid # 85747 ; http://n2t.net/addgene:85747 ; RRID:Addgene_85747) -
For your References section:
PBRM1 Regulates the Expression of Genes Involved in Metabolism and Cell Adhesion in Renal Clear Cell Carcinoma. Chowdhury B, Porter EG, Stewart JC, Ferreira CR, Schipma MJ, Dykhuizen EC. PLoS One. 2016 Apr 21;11(4):e0153718. doi: 10.1371/journal.pone.0153718. eCollection 2016. 10.1371/journal.pone.0153718 PubMed 27100670
Map uploaded by the depositor.
