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Addgene

TetO-FUW-PBRM1-pgk-puro
(Plasmid #85746)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85746 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    FUW
  • Backbone manufacturer
    Rudolf Jaenisch
  • Backbone size w/o insert (bp) 9537
  • Total vector size (bp) 14454
  • Modifications to backbone
    puro resistance added
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    prone to rearrangement
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PBRM1
  • Alt name
    Polybromo-1, BAF180
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4902
  • Entrez Gene
    PBRM1 (a.k.a. BAF180, PB1, RCC, SMARCH1)
  • Promoter CMV
  • Tag / Fusion Protein
    • none

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer fuwF
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TetO-FUW-PBRM1-pgk-puro was a gift from Emily Dykhuizen (Addgene plasmid # 85746 ; http://n2t.net/addgene:85746 ; RRID:Addgene_85746)
  • For your References section:

    PBRM1 Regulates the Expression of Genes Involved in Metabolism and Cell Adhesion in Renal Clear Cell Carcinoma. Chowdhury B, Porter EG, Stewart JC, Ferreira CR, Schipma MJ, Dykhuizen EC. PLoS One. 2016 Apr 21;11(4):e0153718. doi: 10.1371/journal.pone.0153718. eCollection 2016. 10.1371/journal.pone.0153718 PubMed 27100670