TetO-FUW-PBRM1-pgk-puro
(Plasmid #85746)

• Purpose: tetracyline-inducible lentiviral vector expressing human polybromo-1 (PBRM1) in mammlian cells
• Depositing Lab: Emily Dykhuizen
• Publication: Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. doi: 10.1371/journal.pone.0153718. eCollection 2016.

Backbone

• Vector backbone: FUW
• Backbone manufacturer: Rudolf Jaenisch
• Backbone size w/o insert (bp): 9537
• Total vector size (bp): 14454
• Modifications to backbone: puro resistance added
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Growth instructions: prone to rearrangement
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PBRM1
• Alt name: Polybromo-1, BAF180
• Species: H. sapiens (human)
• Insert Size (bp): 4902
• Entrez Gene: PBRM1 (a.k.a. BAF180, PB1, RCC, SMARCH1)
• Promoter: CMV
• Tag / Fusion Protein:
  • none

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: fuwF

Resource Information

• Supplemental Documents:
  • TetO-FUW-PBRM1.gb
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry