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Addgene

AAV-shRNA-Tet2
(Plasmid #85743)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85743 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    shRNA Tet2
  • gRNA/shRNA sequence
    5’-GGATGTAAGTTTGCCAGAAGC-3’
  • Species
    M. musculus (mouse)
  • Tag / Fusion Protein
    • EYFP (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-shRNA-Tet2 was a gift from Hongjun Song (Addgene plasmid # 85743 ; http://n2t.net/addgene:85743 ; RRID:Addgene_85743)

  • For your References section:

    Tet3 regulates synaptic transmission and homeostatic plasticity via DNA oxidation and repair. Yu H, Su Y, Shin J, Zhong C, Guo JU, Weng YL, Gao F, Geschwind DH, Coppola G, Ming GL, Song H. Nat Neurosci. 2015 Jun;18(6):836-43. doi: 10.1038/nn.4008. Epub 2015 Apr 27. 10.1038/nn.4008 PubMed 25915473
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