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Purposeexpresses CFP-FLAG-tagged NS1 monobody.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 85739 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepECFP
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameFLAG-tagged NS1 monobody
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SpeciesH. sapiens (human)
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Insert Size (bp)333
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Tags
/ Fusion Proteins
- ECFP (N terminal on insert)
- Flag (N terminal on insert)
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe NS1 sequence was originally isolated by Dr. Shohei Koide.
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CFP-NS1 was a gift from John O'Bryan (Addgene plasmid # 85739 ; http://n2t.net/addgene:85739 ; RRID:Addgene_85739) -
For your References section:
Inhibition of RAS function through targeting an allosteric regulatory site. Spencer-Smith R, Koide A, Zhou Y, Eguchi RR, Sha F, Gajwani P, Santana D, Gupta A, Jacobs M, Herrero-Garcia E, Cobbert J, Lavoie H, Smith M, Rajakulendran T, Dowdell E, Okur MN, Dementieva I, Sicheri F, Therrien M, Hancock JF, Ikura M, Koide S, O'Bryan JP. Nat Chem Biol. 2016 Nov 7. doi: 10.1038/nchembio.2231. 10.1038/nchembio.2231 PubMed 27820802
Map uploaded by the depositor.
