delNC C-His Ana GvpC in pET28a
(Plasmid #85736)

• Purpose: Expresses a truncated His-tagged GvpC from Anabaena flos-aquae without the N-and C-terminal regions in E.coli
• Depositing Lab: Mikhail Shapiro
• Publication: Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi: 10.1021/acsnano.6b03364. Epub 2016 Jun 30.

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5206
• Total vector size (bp): 5737
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Gas vesicle protein C (truncated and his-tagged)
• Alt name: delNC GvpC
• Species: Anabaena flos-aquae
• Insert Size (bp): 531
• Mutation: Deletion of N and C terminal regions, addition of glycine after the start codon to keep the NcoI site without changing the translation frame, 3 amino acid linker between truncated GvpC and His-tag
• Promoter: T7
• Tag / Fusion Protein:
  • His-tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 terminator reverse

Resource Information

• Supplemental Documents:
  • Addgene delNC C-His Ana GvpC in pET28a .gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry