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pET28b-Ets1-ETS
(Plasmid #85735)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85735 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5231
  • Total vector size (bp) 377
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21*(DE3) for expression and induce at 30°C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    DNA-binding (ETS) domain of murine Ets-1, residues 331 to 440
  • Alt name
    E26 avian leukemia oncogene 1, 5' domain
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    377
  • GenBank ID
    NP_035938.2
  • Entrez Gene
    Ets1 (a.k.a. D230050P06, Ets-1, Tpl1, p54, vs)
  • Tag / Fusion Protein
    • 6xHis following thrombin cleavage site LVPR|G (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note that the DNA sequence in this plasmid is PCRed from the human gene; however, the human and mouse ETS domains are identical at the amino acid level.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-Ets1-ETS was a gift from Gregory Poon (Addgene plasmid # 85735 ; http://n2t.net/addgene:85735 ; RRID:Addgene_85735)

  • For your References section:

    Mechanistic heterogeneity in site recognition by the structurally homologous DNA-binding domains of the ETS family transcription factors Ets-1 and PU.1. Wang S, Linde MH, Munde M, Carvalho VD, Wilson WD, Poon GM. J Biol Chem. 2014 Aug 1;289(31):21605-16. doi: 10.1074/jbc.M114.575340. Epub 2014 Jun 21. 10.1074/jbc.M114.575340 PubMed 24952944
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