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pET28b-PU.1-ETS
(Plasmid #85734)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85734 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET28b
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5246
  • Total vector size (bp) 5612
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Use BL21*(DE3) for expression and induce at 30°C
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ETS domain of murine PU.1 (residues 167-272)
  • Alt name
    Spi1
  • Alt name
    spleen focus forming virus (SFFV) proviral integration oncogene
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Insert Size (bp)
    367
  • GenBank ID
    NM_011355.2 NP_035485.1
  • Entrez Gene
    Spi1 (a.k.a. Dis-1, Dis1, PU.1, Sfpi-1, Sfpi1, Spi-1, Tcfpu1, Tfpu.1)
  • Tag / Fusion Protein
    • 6xHis following thrombin cleavage site LVPR|G (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminator
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b-PU.1-ETS was a gift from Gregory Poon (Addgene plasmid # 85734 ; http://n2t.net/addgene:85734 ; RRID:Addgene_85734)

  • For your References section:

    Mechanistic heterogeneity in site recognition by the structurally homologous DNA-binding domains of the ETS family transcription factors Ets-1 and PU.1. Wang S, Linde MH, Munde M, Carvalho VD, Wilson WD, Poon GM. J Biol Chem. 2014 Aug 1;289(31):21605-16. doi: 10.1074/jbc.M114.575340. Epub 2014 Jun 21. 10.1074/jbc.M114.575340 PubMed 24952944
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