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Addgene

pGCS-N5(GST)
(Plasmid #85722)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85722 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+ F/A
  • Vector type
    mammalian/avian/xenopus/zebrafish
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGCS-N5(GST) was a gift from Hai-Ning Du (Addgene plasmid # 85722 ; http://n2t.net/addgene:85722 ; RRID:Addgene_85722)

  • For your References section:

    Construction of a series of pCS2+ backbone-based Gateway vectors for overexpressing various tagged proteins in vertebrates. Wang HY, Li Y, Xue T, Cheng N, Du HN. Acta Biochim Biophys Sin (Shanghai). 2016 Oct 26. 10.1093/abbs/gmw107 PubMed 27797719
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