pHA2
(Plasmid #85710)

• Purpose: (Empty Backbone) This plasmid provides a BAC vector backbone containing GFP, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes
• Depositing Labs: Ulrich Koszinowski, Martin Messerle
• Publication: Adler et al J Virol. 2000 Aug;74(15):6964-74.

Backbone

• Vector backbone: pK18
• Vector type: Bacterial Expression, Cre/Lox ; Herpesvirus Cloning
• Selectable markers: gpt (E.coli Xanthine-guanine phosphoribosyl transferase gene); select with xanthine and mycophenolic acid

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 25 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

he BAC vector is derived from the E.coli F plasmid. The BAC vector was cloned into the high copy vector pK18, to increse yields. The BAC vector is typically excised by PacI. The BAC vector contains genes for participation (sopA, B,C, a repE gene (required for replication) and an origin of replication (oriS). There is also a chloramphenicol resistance gene. The BAC vector carries a gpt gene, driven by the HSV tk promoter; the gpt gene is followed by the SV40 polyA signal sequence (ca 125 bp). The BAC vector is terminally flanked by loxP sites. The BAC vector seqeunces also carry the EGFP gene, under control of the HCMV MIEP, followed by SV40 polyA signal sequence. The plasmid contains also a 1.5 kb EcoRI-fragment from the murine herpesvirus MHV68. The sequence of pK18 is available from Genbank ACCESSION M17626.