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Addgene

pTKv10.2
(Plasmid #85706)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 85706 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTracetCMV
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KCNH5
  • Alt name
    Eag2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Entrez Gene
    KCNH5 (a.k.a. EAG2, H-EAG2, Kv10.2, hEAG2)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer BGH-rev
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTKv10.2 was a gift from Luis Pardo (Addgene plasmid # 85706 ; http://n2t.net/addgene:85706 ; RRID:Addgene_85706)

  • For your References section:

    Monoclonal antibody blockade of the human Eag1 potassium channel function exerts antitumor activity. Gomez-Varela D, Zwick-Wallasch E, Knotgen H, Sanchez A, Hettmann T, Ossipov D, Weseloh R, Contreras-Jurado C, Rothe M, Stuhmer W, Pardo LA. Cancer Res. 2007 Aug 1;67(15):7343-9. 10.1158/0008-5472.CAN-07-0107 PubMed 17671204
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